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dreadd agonist compound 21  (Tocris)


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    Structured Review

    Tocris dreadd agonist compound 21
    Dreadd Agonist Compound 21, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/compounds+21/DREADD+agonist+21/10__1038_slash_s41598___026___71111___2-78-22-31
    Average 95 stars, based on 41 article reviews
    dreadd agonist compound 21 - by Bioz Stars, 2026-11
    95/100 stars

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    other:

    Article Title: PIEZO1 transduces mechanical itch in mice
    Article Snippet: For chemical itch experiments, compounds (histamine dihydrochloride: 50 μg in PBS, Tocris; Yoda1: 14.2 ng, 142 ng or 355 ng in 1% DMSO-PBS, Tocris; chloroquine diphosphate: 200 μg in PBS, Tocris; recombinant mouse IL-31: 60 pmol in 0.9% NaCl, Peprotech; DREADD agonist 21: 25 μg in PBS, Tocris) were injected via the intradermal route using a 31g insulin syringe in a total volume of 20 μl.

    Article Title: The GPR39 Receptor Plays an Important Role in the Pathogenesis of Overactive Bladder and Corticosterone-Induced Depression
    Article Snippet: The experiment utilized the following compounds: CORT ((11β)-11,21-Dihydroxypregn-4-ene-3,20-dione), obtained from Tocris Bioscience in Bristol, Great Britain, was administered subcutaneously at 20 mg/kg daily for a two-week period.

    Article Title: The GPR39 Receptor Plays an Important Role in the Pathogenesis of Overactive Bladder and Corticosterone-Induced Depression
    Article Snippet: The experiment utilized the following compounds: CORT ((11β)-11,21-Dihydroxypregn4-ene-3,20-dione), obtained from Tocris Bioscience in Bristol, Great Britain, was administered subcutaneously at 20 mg/kg daily for a two-week period.

    Emulsion:

    Article Title: Glutamate transporter Slc1a3 mediates inter‐niche stem cell activation during skin growth
    Article Snippet: .. The following compounds were applied: DL‐TBOA (21 mM in DMSO; Tocris) and MTEP (15 mM in DMSO; Abcam). l ‐Glutamate (2.5%; l ‐glutamic acid; Sigma) was applied in a cutaneous emulsion (Propyless, containing 20% propylene glycol and 9% fat; Merck Sharp & Dome). ..

    High Throughput Screening Assay:

    Article Title: A highly potent bi-thiazole inhibitor of LOX rewires collagen architecture and enhances chemoresponse in triple-negative breast cancer.
    Article Snippet: .. A high throughput drug-like molecule library screen combined with structure-activity relationship (SAR) analyses identify bi-thiazole compounds as LOX inhibitors To identify potent and safe LOX inhibitors, a high-throughput screen (HTS) with a cell-based LOX activity assay was performed with more than 5,000 drug-like compounds from the EXPRESSPick diversified library (4,480 molecules, ChemBridge) and Tocriscreen library of bioactive compounds (1,280 compounds, 2 Cell Chemical Biology 31, 1–16, November 21, 2024 Tocris), each at a single concentration of 10 mM using the MDAMB-231 TNBC cell line (Figures 1A and 1B). .. This screen identified 25 compounds from the ChemBridge library and 56 hits from the Tocris library that successfully inhibited R90% of cellular LOX activity.

    Activity Assay:

    Article Title: A highly potent bi-thiazole inhibitor of LOX rewires collagen architecture and enhances chemoresponse in triple-negative breast cancer.
    Article Snippet: .. A high throughput drug-like molecule library screen combined with structure-activity relationship (SAR) analyses identify bi-thiazole compounds as LOX inhibitors To identify potent and safe LOX inhibitors, a high-throughput screen (HTS) with a cell-based LOX activity assay was performed with more than 5,000 drug-like compounds from the EXPRESSPick diversified library (4,480 molecules, ChemBridge) and Tocriscreen library of bioactive compounds (1,280 compounds, 2 Cell Chemical Biology 31, 1–16, November 21, 2024 Tocris), each at a single concentration of 10 mM using the MDAMB-231 TNBC cell line (Figures 1A and 1B). .. This screen identified 25 compounds from the ChemBridge library and 56 hits from the Tocris library that successfully inhibited R90% of cellular LOX activity.

    Concentration Assay:

    Article Title: A highly potent bi-thiazole inhibitor of LOX rewires collagen architecture and enhances chemoresponse in triple-negative breast cancer.
    Article Snippet: .. A high throughput drug-like molecule library screen combined with structure-activity relationship (SAR) analyses identify bi-thiazole compounds as LOX inhibitors To identify potent and safe LOX inhibitors, a high-throughput screen (HTS) with a cell-based LOX activity assay was performed with more than 5,000 drug-like compounds from the EXPRESSPick diversified library (4,480 molecules, ChemBridge) and Tocriscreen library of bioactive compounds (1,280 compounds, 2 Cell Chemical Biology 31, 1–16, November 21, 2024 Tocris), each at a single concentration of 10 mM using the MDAMB-231 TNBC cell line (Figures 1A and 1B). .. This screen identified 25 compounds from the ChemBridge library and 56 hits from the Tocris library that successfully inhibited R90% of cellular LOX activity.



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    (A) Design of the experiment. CNO, clozapine-N-oxide (150 mg/kg); IP, intraperitoneal; V2, maxillary area; V, von Frey test. (B) The effects of chemogenetic silencing of brushing-activated TRAPed trigeminal ganglia (BA TG) neurons on punctate allodynia. t(6.3) = 6.07; * p < 0.05 in Dunnett post hoc test following two-way ANOVA. (C) In a separate cohort of mice, tamoxifen was administered but face brushing was not performed. CNO at 24 days did not produce analgesia. (D) Design of the experiment. W, nocifensive face wiping behaviors; C21, compound 21 (non-hydrolyzable analogue of clozapine-N-oxide; 1 mmol in 20 mL); V2, maxillary area. (E) Face wiping behaviors were quantified for 20 min following the injection of saline or C21 to V2 skin. t(34) = 2.8, * p < 0.05 in Sidak post hoc testing following two-way ANOVA. n = 5 in mCherry and 6 in hM3Dq. In (B), (C), and (E), the error bars indicate SEM.

    Journal: Cell reports

    Article Title: Genetic identification of mouse trigeminal afferents responsible for mechanical allodynia

    doi: 10.1016/j.celrep.2025.116803

    Figure Lengend Snippet: (A) Design of the experiment. CNO, clozapine-N-oxide (150 mg/kg); IP, intraperitoneal; V2, maxillary area; V, von Frey test. (B) The effects of chemogenetic silencing of brushing-activated TRAPed trigeminal ganglia (BA TG) neurons on punctate allodynia. t(6.3) = 6.07; * p < 0.05 in Dunnett post hoc test following two-way ANOVA. (C) In a separate cohort of mice, tamoxifen was administered but face brushing was not performed. CNO at 24 days did not produce analgesia. (D) Design of the experiment. W, nocifensive face wiping behaviors; C21, compound 21 (non-hydrolyzable analogue of clozapine-N-oxide; 1 mmol in 20 mL); V2, maxillary area. (E) Face wiping behaviors were quantified for 20 min following the injection of saline or C21 to V2 skin. t(34) = 2.8, * p < 0.05 in Sidak post hoc testing following two-way ANOVA. n = 5 in mCherry and 6 in hM3Dq. In (B), (C), and (E), the error bars indicate SEM.

    Article Snippet: Compound 21 dihydrochloride (C21) , Hello Bio , HB6124.

    Techniques: Injection, Saline